Pemetaan Enzim Restriksi Gen KiSS1 pada Kambing
Keywords:
Kambing, KiSS1, Primer3, Pemetaan enzim restriksi, BioEdit
Abstract
Enzim restriksi memiliki peran penting dalam menentukan keragaman dan fungsi in vivo. Penelitian ini bertujuan untuk memetakan enzim restriksi pada gen KiSS1 kambing. Materi yang digunakan pada penelitian ini adalah data sekuen gen KiSS1 kambing yang tersedia pada NCBI. Desain primer dilakukan menggunakan program Primer3. Primer digunakan untuk mengapit target gen KiSS1 yang dipetakan enzim restriksinya. Pada penelitian ini ditemukan 3 enzim yaitu BsrB1 (SNP g.505A/T), FatI dan NlaIII (SNP g.1608G/A). Ketiga enzim tersebut dapat direkomendasikan untuk penelitian selanjutnya yaitu genotyping menggunakan metode PCR-RFLP.
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References
Hall, T., I. Biosciences & C. Carlsbad. 2011. BioEdit: an important software for molecular biology. GERF Bull Biosci 2:60–61.
Latifah, L., D. Maharani, A. Kustantinah & T. Hartatik. 2018. Association of melanocortin 4 receptor gene polymorphism with growth traits in Bligon goat.. J. Indones. Trop. Anim. Agric. 43:343–351.
Latifah, D.A. Priyadi, D. Maharani, Kustantinah & T. Hartatik. 2017. Genetic analysis using partial sequencing of melanocortin 4 receptor (MC4R) gene in Bligon goat. Media Peternak. 40:71–77. doi:10.5398/medpet.2017.40.2.71.
Li, D., W. Yu & M. Liu. 2009. Regulation of KiSS1 gene expression. Peptides 30:130–138.
Maitra, A., R. Sharma, S. Ahlawat, M.S. Tantia, M. Roy & V. Prakash. 2014. Association analysis of polymorphisms in caprine KiSS1 gene with reproductive traits. Anim. Reprod. Sci. 151:71–77.
Mekuriaw, G., J.M. Mwacharo, T. Dessie, O. Mwai, A. Djikeng, S. Osama, G. Gebreyesus, A. Kidane, S. Abegaz & K. Tesfaye. 2017. Polymorphism analysis of kisspeptin (KISS1) gene and its association with litter size in Ethiopian indigenous goat populations. African J. Biotechnol. 16:1254–1264.
Sahoo, S.S., C. Mishra, R. Kaushik, P.K. Rout, M.K. Singh, S. Bhusan & M.S. Dige. 2019. Association of a SNP in KISS 1 gene with reproductive traits in goats. Biol. Rhythm Res. 1–12.
Sankhyan, V., Y.P. Thakur & P.K. Dogra. 2019. Association of kisspeptin gene (KISS1) with litter size in migratory Gaddi goats in western Himalayan state of Himachal Pradesh. Indian J. Anim. Sci. 89:1352–1355.
Untergasser, A., I. Cutcutache, T. Koressaar, J. Ye, B.C. Faircloth, M. Remm & S.G. Rozen. 2012. Primer3—new capabilities and interfaces. Nucleic Acids Res. 40:e115–e115.
Williams, R.J. 2003. Restriction endonuclease. Mol. Biotechnol. 23:225–243.
Latifah, L., D. Maharani, A. Kustantinah & T. Hartatik. 2018. Association of melanocortin 4 receptor gene polymorphism with growth traits in Bligon goat.. J. Indones. Trop. Anim. Agric. 43:343–351.
Latifah, D.A. Priyadi, D. Maharani, Kustantinah & T. Hartatik. 2017. Genetic analysis using partial sequencing of melanocortin 4 receptor (MC4R) gene in Bligon goat. Media Peternak. 40:71–77. doi:10.5398/medpet.2017.40.2.71.
Li, D., W. Yu & M. Liu. 2009. Regulation of KiSS1 gene expression. Peptides 30:130–138.
Maitra, A., R. Sharma, S. Ahlawat, M.S. Tantia, M. Roy & V. Prakash. 2014. Association analysis of polymorphisms in caprine KiSS1 gene with reproductive traits. Anim. Reprod. Sci. 151:71–77.
Mekuriaw, G., J.M. Mwacharo, T. Dessie, O. Mwai, A. Djikeng, S. Osama, G. Gebreyesus, A. Kidane, S. Abegaz & K. Tesfaye. 2017. Polymorphism analysis of kisspeptin (KISS1) gene and its association with litter size in Ethiopian indigenous goat populations. African J. Biotechnol. 16:1254–1264.
Sahoo, S.S., C. Mishra, R. Kaushik, P.K. Rout, M.K. Singh, S. Bhusan & M.S. Dige. 2019. Association of a SNP in KISS 1 gene with reproductive traits in goats. Biol. Rhythm Res. 1–12.
Sankhyan, V., Y.P. Thakur & P.K. Dogra. 2019. Association of kisspeptin gene (KISS1) with litter size in migratory Gaddi goats in western Himalayan state of Himachal Pradesh. Indian J. Anim. Sci. 89:1352–1355.
Untergasser, A., I. Cutcutache, T. Koressaar, J. Ye, B.C. Faircloth, M. Remm & S.G. Rozen. 2012. Primer3—new capabilities and interfaces. Nucleic Acids Res. 40:e115–e115.
Williams, R.J. 2003. Restriction endonuclease. Mol. Biotechnol. 23:225–243.
Published
2020-11-02
Section
Articles
